Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia
Identifieur interne : 005A77 ( Main/Exploration ); précédent : 005A76; suivant : 005A78Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia
Auteurs : Patrick C. Y. Woo [Hong Kong] ; Susanna K. P. Lau [Hong Kong] ; Hoi-Wah Tsoi [Hong Kong] ; Kwok-Hung Chan [Hong Kong] ; Beatrice H. L. Wong [Hong Kong] ; Xiao-Yan Che [République populaire de Chine] ; Victoria K. P. Tam [Hong Kong] ; Sidnev C. F. Tam [Hong Kong] ; Vincent C. C. Cheng [Hong Kong] ; Ivan F. N. Hung [Hong Kong] ; Samson S. Y. Wong [Hong Kong] ; Bo-Jian Zheng [Hong Kong] ; YI GUAN [Hong Kong] ; Kwok-Yung Yuen [Hong Kong]Source :
- Lancet : (British edition) [ 0140-6736 ] ; 2004.
Descripteurs français
- KwdFr :
- Chine (épidémiologie), Coronavirus (génétique), Coronavirus (isolement et purification), Donneurs de sang, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (analyse), Humains, Immunoglobuline G (analyse), Immunoglobuline G (immunologie), Infection croisée (immunologie), Infection croisée (épidémiologie), Pneumopathie virale (immunologie), Pneumopathie virale (virologie), Pneumopathie virale (épidémiologie), Protéines de l'enveloppe virale (analyse), Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Protéines recombinantes (analyse), Protéines recombinantes (immunologie), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Syndrome respiratoire aigu sévère (épidémiologie), Technique de Western, Test ELISA, Études séroépidémiologiques.
- MESH :
- analyse : Glycoprotéines membranaires, Immunoglobuline G, Protéines de l'enveloppe virale, Protéines recombinantes.
- génétique : Coronavirus, Protéines nucléocapside.
- immunologie : Immunoglobuline G, Infection croisée, Pneumopathie virale, Protéines nucléocapside, Protéines recombinantes, Syndrome respiratoire aigu sévère.
- isolement et purification : Coronavirus.
- virologie : Pneumopathie virale, Syndrome respiratoire aigu sévère.
- épidémiologie : Chine, Infection croisée, Pneumopathie virale, Syndrome respiratoire aigu sévère.
- Pascal (Inist)
- Donneurs de sang, Glycoprotéine de spicule des coronavirus, Humains, Infection, Taux, Coronavirus, Pneumonie, Epidémiologie, Epidémie, Hong Kong, Technique de Western, Test ELISA, Virose, Diagnostic différentiel, Génome, Homme, Santé publique, Syndrome respiratoire aigu sévère, Études séroépidémiologiques.
- Wicri :
- geographic : Hong Kong, République populaire de Chine.
- topic : Homme, Santé publique.
English descriptors
- KwdEn :
- Blood Donors, Blotting, Western, China (epidemiology), Coronavirus, Coronavirus (genetics), Coronavirus (isolation & purification), Cross Infection (epidemiology), Cross Infection (immunology), Differential diagnostic, Enzyme-Linked Immunosorbent Assay, Epidemic, Epidemiology, Genome, Hong Kong, Human, Humans, Immunoglobulin G (analysis), Immunoglobulin G (immunology), Infection, Membrane Glycoproteins (analysis), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Pneumonia, Pneumonia, Viral (epidemiology), Pneumonia, Viral (immunology), Pneumonia, Viral (virology), Public health, Rate, Recombinant Proteins (analysis), Recombinant Proteins (immunology), Seroepidemiologic Studies, Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Severe acute respiratory syndrome, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (analysis), Viral disease.
- MESH :
- chemical , analysis : Immunoglobulin G, Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- geographic , epidemiology : China.
- epidemiology : Cross Infection, Pneumonia, Viral, Severe Acute Respiratory Syndrome.
- genetics : Coronavirus, Nucleocapsid Proteins.
- immunology : Cross Infection, Immunoglobulin G, Nucleocapsid Proteins, Pneumonia, Viral, Recombinant Proteins, Severe Acute Respiratory Syndrome.
- isolation & purification : Coronavirus.
- virology : Pneumonia, Viral, Severe Acute Respiratory Syndrome.
- Blood Donors, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Humans, Seroepidemiologic Studies, Spike Glycoprotein, Coronavirus.
Abstract
Background Although the genome of severe acute respiratory syndrome coronavirus (SARS-CoV) has been sequenced and a possible animal reservoir identified, seroprevalence studies and mass screening for detection of subclinical and non-pneumonic infections are still lacking. Methods We cloned and purified the nucleocapsid protein and spike polypeptide of SARS-CoV and examined their immunogenicity with serum from patients with SARS-CoV pneumonia. An ELISA based on recombinant nucleocapsid protein for IgG detection was tested with serum from 149 healthy blood donors who donated 3 years previously and with serum positive for antibodies against SARS-CoV (by indirect immunofluorescence assay) from 106 patients with SARS-CoV pneumonia. The seroprevalence of SARS-CoV was studied with the ELISA in healthy blood donors who donated during the SARS outbreak in Hong Kong, non-pneumonic hospital inpatients, and symptom-free health-care workers. All positive samples were confirmed by two separate western-blot assays (with recombinant nucleocapsid protein and recombinant spike polypeptide). Findings Western-blot analysis showed that the nucleocapsid protein and spike polypeptide of SARS-CoV are highly immunogenic. The specificity of the IgG antibody test (ELISA with positive samples confirmed by the two western-blot assays) was 100%, and the sensitivity was 94.3%. Three of 400 healthy blood donors who donated during the SARS outbreak and one of 131 non-pneumonic paediatric inpatients were positive for IgG antibodies, confirmed by the two western-blot assays (total, 0.48% of our study population). Interpretation Our findings support the existence of subclinical or non-pneumonic SARS-CoV infections. Such infections are more common than SARS-CoV pneumonia in our locality.
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Le document en format XML
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Y." last="Woo">Patrick C. Y. Woo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K. P." last="Lau">Susanna K. P. Lau</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Tsoi, Hoi Wah" sort="Tsoi, Hoi Wah" uniqKey="Tsoi H" first="Hoi-Wah" last="Tsoi">Hoi-Wah Tsoi</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Wong, Beatrice H L" sort="Wong, Beatrice H L" uniqKey="Wong B" first="Beatrice H. L." last="Wong">Beatrice H. L. Wong</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Central Laboratory, Zhujiang Hospital, First Military Medical University</s1><s2>Guangzhou</s2><s3>CHN</s3><sZ>6 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Tam, Victoria K P" sort="Tam, Victoria K P" uniqKey="Tam V" first="Victoria K. P." last="Tam">Victoria K. P. Tam</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Tam, Sidnev C F" sort="Tam, Sidnev C F" uniqKey="Tam S" first="Sidnev C. F." last="Tam">Sidnev C. F. Tam</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Clinical Biochemistry, Queen Mary Hospital</s1><s3>HKG</s3><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Clinical Biochemistry, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Vincent C C" sort="Cheng, Vincent C C" uniqKey="Cheng V" first="Vincent C. C." last="Cheng">Vincent C. C. Cheng</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Hung, Ivan F N" sort="Hung, Ivan F N" uniqKey="Hung I" first="Ivan F. N." last="Hung">Ivan F. N. Hung</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Wong, Samson S Y" sort="Wong, Samson S Y" uniqKey="Wong S" first="Samson S. Y." last="Wong">Samson S. Y. Wong</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Yi Guan" sort="Yi Guan" uniqKey="Yi Guan" last="Yi Guan">YI GUAN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</s1><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microblology, University of Hong Kong, University Pathology Building, Queen Mary Hospital</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Lancet : (British edition)</title><title level="j" type="abbreviated">Lancet : (Br. ed.)</title><idno type="ISSN">0140-6736</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Lancet : (British edition)</title><title level="j" type="abbreviated">Lancet : (Br. ed.)</title><idno type="ISSN">0140-6736</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blood Donors</term><term>Blotting, Western</term><term>China (epidemiology)</term><term>Coronavirus</term><term>Coronavirus (genetics)</term><term>Coronavirus (isolation & purification)</term><term>Cross Infection (epidemiology)</term><term>Cross Infection (immunology)</term><term>Differential diagnostic</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Epidemic</term><term>Epidemiology</term><term>Genome</term><term>Hong Kong</term><term>Human</term><term>Humans</term><term>Immunoglobulin G (analysis)</term><term>Immunoglobulin G (immunology)</term><term>Infection</term><term>Membrane Glycoproteins (analysis)</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (immunology)</term><term>Pneumonia</term><term>Pneumonia, Viral (epidemiology)</term><term>Pneumonia, Viral (immunology)</term><term>Pneumonia, Viral (virology)</term><term>Public health</term><term>Rate</term><term>Recombinant Proteins (analysis)</term><term>Recombinant Proteins (immunology)</term><term>Seroepidemiologic Studies</term><term>Severe Acute Respiratory Syndrome (epidemiology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Severe acute respiratory syndrome</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (analysis)</term><term>Viral disease</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Chine (épidémiologie)</term><term>Coronavirus (génétique)</term><term>Coronavirus (isolement et purification)</term><term>Donneurs de sang</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (analyse)</term><term>Humains</term><term>Immunoglobuline G (analyse)</term><term>Immunoglobuline G (immunologie)</term><term>Infection croisée (immunologie)</term><term>Infection croisée (épidémiologie)</term><term>Pneumopathie virale (immunologie)</term><term>Pneumopathie virale (virologie)</term><term>Pneumopathie virale (épidémiologie)</term><term>Protéines de l'enveloppe virale (analyse)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (immunologie)</term><term>Protéines recombinantes (analyse)</term><term>Protéines recombinantes (immunologie)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Syndrome respiratoire aigu sévère (épidémiologie)</term><term>Technique de Western</term><term>Test ELISA</term><term>Études séroépidémiologiques</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Immunoglobulin G</term><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Immunoglobuline G</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Cross Infection</term><term>Pneumonia, Viral</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus</term><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Immunoglobuline G</term><term>Infection croisée</term><term>Pneumopathie virale</term><term>Protéines nucléocapside</term><term>Protéines recombinantes</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Cross Infection</term><term>Immunoglobulin G</term><term>Nucleocapsid Proteins</term><term>Pneumonia, Viral</term><term>Recombinant Proteins</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Pneumopathie virale</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Pneumonia, Viral</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Chine</term><term>Infection croisée</term><term>Pneumopathie virale</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blood Donors</term><term>Blotting, Western</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Seroepidemiologic Studies</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Donneurs de sang</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Infection</term><term>Taux</term><term>Coronavirus</term><term>Pneumonie</term><term>Epidémiologie</term><term>Epidémie</term><term>Hong Kong</term><term>Technique de Western</term><term>Test ELISA</term><term>Virose</term><term>Diagnostic différentiel</term><term>Génome</term><term>Homme</term><term>Santé publique</term><term>Syndrome respiratoire aigu sévère</term><term>Études séroépidémiologiques</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Hong Kong</term><term>République populaire de Chine</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term><term>Santé publique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background Although the genome of severe acute respiratory syndrome coronavirus (SARS-CoV) has been sequenced and a possible animal reservoir identified, seroprevalence studies and mass screening for detection of subclinical and non-pneumonic infections are still lacking. Methods We cloned and purified the nucleocapsid protein and spike polypeptide of SARS-CoV and examined their immunogenicity with serum from patients with SARS-CoV pneumonia. An ELISA based on recombinant nucleocapsid protein for IgG detection was tested with serum from 149 healthy blood donors who donated 3 years previously and with serum positive for antibodies against SARS-CoV (by indirect immunofluorescence assay) from 106 patients with SARS-CoV pneumonia. The seroprevalence of SARS-CoV was studied with the ELISA in healthy blood donors who donated during the SARS outbreak in Hong Kong, non-pneumonic hospital inpatients, and symptom-free health-care workers. All positive samples were confirmed by two separate western-blot assays (with recombinant nucleocapsid protein and recombinant spike polypeptide). Findings Western-blot analysis showed that the nucleocapsid protein and spike polypeptide of SARS-CoV are highly immunogenic. The specificity of the IgG antibody test (ELISA with positive samples confirmed by the two western-blot assays) was 100%, and the sensitivity was 94.3%. Three of 400 healthy blood donors who donated during the SARS outbreak and one of 131 non-pneumonic paediatric inpatients were positive for IgG antibodies, confirmed by the two western-blot assays (total, 0.48% of our study population). Interpretation Our findings support the existence of subclinical or non-pneumonic SARS-CoV infections. Such infections are more common than SARS-CoV pneumonia in our locality.</div></front></TEI><affiliations><list><country><li>Hong Kong</li><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="Hong Kong"><noRegion><name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C. Y." last="Woo">Patrick C. Y. Woo</name></noRegion><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><name sortKey="Cheng, Vincent C C" sort="Cheng, Vincent C C" uniqKey="Cheng V" first="Vincent C. C." last="Cheng">Vincent C. C. Cheng</name><name sortKey="Hung, Ivan F N" sort="Hung, Ivan F N" uniqKey="Hung I" first="Ivan F. N." last="Hung">Ivan F. N. Hung</name><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K. P." last="Lau">Susanna K. P. Lau</name><name sortKey="Tam, Sidnev C F" sort="Tam, Sidnev C F" uniqKey="Tam S" first="Sidnev C. F." last="Tam">Sidnev C. F. Tam</name><name sortKey="Tam, Victoria K P" sort="Tam, Victoria K P" uniqKey="Tam V" first="Victoria K. P." last="Tam">Victoria K. P. Tam</name><name sortKey="Tsoi, Hoi Wah" sort="Tsoi, Hoi Wah" uniqKey="Tsoi H" first="Hoi-Wah" last="Tsoi">Hoi-Wah Tsoi</name><name sortKey="Wong, Beatrice H L" sort="Wong, Beatrice H L" uniqKey="Wong B" first="Beatrice H. L." last="Wong">Beatrice H. L. Wong</name><name sortKey="Wong, Samson S Y" sort="Wong, Samson S Y" uniqKey="Wong S" first="Samson S. Y." last="Wong">Samson S. Y. Wong</name><name sortKey="Yi Guan" sort="Yi Guan" uniqKey="Yi Guan" last="Yi Guan">YI GUAN</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name></country><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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